Biochemical studies of peanut cells grown in suspension cultures treated with aluminium
1991
Marziah, M. | Abdullah, P. | Mispar, N. | Syed, M.A.
Preliminary studies were conducted to examine the biochemical responses of peanut (Archis hypogaea L. 7920B-3B) cell suspension cultures grown in modified L-6 medium and treated with different of aluminium. Studies on nitrate reductase (EC 1.6.6.1) and glutamine synthetase (EC 6.3.1.2) showed an initial increase in activities of both enzymes with increase in Al supplied but began to decline with higher Al-treatments. Maximum nitrate reductase and glutamine synthetase activity was observed at 1000 micromolar Al and 600 micromolar Al, respectively. The concentration of buffer-soluble protein was higher in Al-treated cell extracts than in the control and the different levels of Al added to the cultures did not significantly change the soluble protein content in each treatment. Results of polyacrylamide gel electrophoresis also showed that a new protein was observed in all Al-treated cell extracts. An increase in phosphate supply did not alleviate the effects of Al-toxicity as indicated by the poor cell growth. The activity of acid phosphatase (EC 3.1.3.2) was inhibited by Al at all levels of phosphate (100-1500 micromolar P) applied. The addition of 2 mM organic acids (isocitrate, fumarate and succinate) to the culture media appeared to overcome the effects of Al toxicity in peanut cell suspension cultures in the following descending order: isocitrate > fumarate > succinate. The addition of citrate did not promote cell growth and development, in fact, a slight inhibitory effect was observed.
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