Microscopic and proteomic analysis of Zea mays roots (P30F53 variety) inoculated with Azospirillum brasilense strain FP2
2015
Faleiro, Alexandro Cézar | Neto, Pedro Alexandre Valentim | de Souza, Thaysi Ventura | Santos, Marisa | Maisonnave Arisi, Ana Carolina
Plant growth-promoting rhizobacteria (PGPR) are considered to have a beneficial effect on host plants. Azospirillum brasilense is a diazotroph associated with important agricultural crops such as maize and wheat, and has been used as model organism to investigate associative plant growth promotion. In this study, we have employed scanning electronic microscopic to observe the presence of bacterium on the root surface. Two-dimensional gel electrophoresis and mass spectrometry were used to analyze the changes in protein profile of maize roots in response to A. brasilense strain FP2 7 days after inoculation and we observed 46 differently expressed protein spots. We report the identification of three of these proteins.
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