Micelle to solvent stacking of two alkaloids in nonaqueous capillary electrophoresis
2011
Zhu, Hua-dong | Lü, Wen-juan | Li, Hong-hong | Ma, Yan-hua | Hu, Shao-qiang | Chen, Hong-li | Chen, Xing-guo
This paper for the first time describes the development of micelle to solvent stacking (MSS) to nonaqueous capillary electrophoresis (NACE). In this proposed MSS-NACE, sodium dodecyl sulfate (SDS) micelles transport, release, and focus analytes from the sample solution to the running buffer using methanol as their solvent. After the focusing step, the focused analytes were separated via NACE. The focusing mechanism and influencing factors were discussed using berberine (BBR) and jatrorrhizine (JTZ) as model compounds. And the optimum condition was obtained as following: 50mM ammonium acetate, 6% (v/v) acetic acid and 10mM SDS in redistilled water as sample matrix, 50mM ammonium acetate and 6% (v/v) acetic acid in pure methanol as the running buffer, −20kV focusing voltage with 30min focusing time. Under these conditions, this method afforded limits of detection (S/N=3) of 0.002μg/mL and 0.003μg/mL for BBR and JTZ, respectively. In contrast to conventional NACE, the concentration sensitivity was improved 128–153-fold.
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