Biological control ginseng grey mold and plant colonization by antagonistic bacteria isolated from rhizospheric soil of Panax ginseng Meyer
2019
Sun, Zhuo | Yang, Limin | Han, Mei | Han, Zhong-ming | Yang, Li | Cheng, Lin | Zhang, Tao | Lv, Ze-liang
Botrytis cinerea Pers.ex Fr. is one of the common soil-borne and fungal pathogens in economic crop such as ginseng worldwide. One ideal strategy for controlling the pathogen B. cinerea is to develop and use beneficial rhizobacteria. This study aims to assess the potential of rhizospheric bacteria as antifungal agents against ginseng grey mold disease. In this study, 169 bacterial strains isolated from rhizospheric soil of ginseng plants were screened using the K-B paper dispersion and the Oxford cup plate assay, and strain SW-34 was selected as a potential stock for biocontrol due to the broad-spectrum antibiotic activity of the SW-34 fermentation liquor against pathogens. To assess the antifungal efficacy of SW-34 against ginseng grey mold, ginseng growth promotion and colonization ability of SW-34, pot experiments were performed under plastic greenhouse conditions. Morphology, physiological and biochemical characteristics, 16S rRNA and gyrB gene sequences were performed for identifying taxonomic characterization of strain SW-34. The results showed that, SW-34 (which was identified as Bacillus amyloliquefaciens) displayed antagonistic activity against B. cinerea, of 85.13%, and also provided broad-spectrum antibiotic activity. Inoculation of the SW-34 bacterial cell suspension suppressed disease lesions of ginseng grey mold with strong control efficacy of 72.29% and was significantly higher than that of the Mancozeb 70% WP fungicide treatment (P<0.05). Moreover, SW-34 not only exhibited higher population levels in the ginseng plant (root, leaf and stem) but also caused ginseng plant growth promotion. B. amyloliquefaciens SW-34 inhibited ginseng grey mold successfully. Thus, our study provides a basis for the potential biocontrol of ginseng grey mold resulting from B. cinerea.
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