Separation of phosphatidylcholine and its molecular species by high-performance liquid chromatography
1990
Glass, R.L.
Isocratic separation of phosphatidylcholine (PC) and PC molecular species from a soybean leaf lipidic extract using high-performance liquid chromatography (HPLC) is described. PC separation was achieved within 20 min on a semipreparative silica normal phase column using isooctane/2-propanol/water (40:53:7 v/v/v). As much as 1.25 mg of PC was obtained after a single injection. Individual molecular species of PC were separated on an analytical C18 reversed-phase column using methanol/0.1 M ammonium acetate (95:5 v/v, pH 7.4). Spectrophotometric detection of PC and molecular species was made at 205 nm.
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