NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoKATP channel opening in rat hippocampal neurons

Yamanaka, Ryu; Shindo, Yutaka; Hotta, Kohji; Suzuki, Kōji; Oka, Kotaro

NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoKATP channel opening in rat hippocampal neurons

2013

Yamanaka, Ryu | Shindo, Yutaka | Hotta, Kohji | Suzuki, Kōji | Oka, Kotaro

Intracellular Mg2+ concentration ([Mg2+]i) and NO regulate cell survival and death. To reveal the involvement of NO in intracellular Mg2+ regulation, we visualized intracellular Mg2+ using the fluorescent Mg2+ indicator KMG-104-AM in rat hippocampal neurons. Pharmacological experiments using SNAP, 8-Br-cGMP, diazoxide and several inhibitors revealed that the NO/cGMP/Protein kinsase G (PKG) signaling pathway triggers an increase in [Mg2+]i, and that Mg2+ mobilization is due to Mg2+ release from mitochondria induced by mitoKATP channel opening. In addition, Mg2+ release is potentiated by the positive feedback loop including mitoKATP channel opening, mitochondrial depolarization and PKC activation.

Show more [+]

AGROVOC Keywords

fluorescence magnesium mitochondria mitochondria nitric oxide rats

Bibliographic information

Published in

FEBS letters

Volume 587 Issue 16 Pagination 2643 - 2648 ISSN 0014-5793

Publisher

Elsevier B.V.

Other Subjects

Mitokatp channel; Signal transduction; Kmg-104; Δψm; Pkc; Hippocampal neuron; Cgmp-dependent protein kinase; Protein kinase c; Neurons; [mg2+]i; Pkg; Ros; Mg2+; [ca2+]i; Cyclic gmp; Cell viability; Diazoxide

Language

English

Type

Text; Journal Article

In AGRIS since: 2024-02-28

Format: MODS

Data Provider

This bibliographic record has been provided by National Agricultural Library

Discover this data provider's collection in AGRIS

Links

DOI https://dx.doi.org/10.1016/j.febslet.2013.06.049

Lookup at Google Scholar