NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoKATP channel opening in rat hippocampal neurons

Yamanaka, Ryu; Shindo, Yutaka; Hotta, Kohji; Suzuki, Kōji; Oka, Kotaro

NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoKATP channel opening in rat hippocampal neurons

2013

Yamanaka, Ryu | Shindo, Yutaka | Hotta, Kohji | Suzuki, Kōji | Oka, Kotaro

Intracellular Mg2+ concentration ([Mg2+]i) and NO regulate cell survival and death. To reveal the involvement of NO in intracellular Mg2+ regulation, we visualized intracellular Mg2+ using the fluorescent Mg2+ indicator KMG-104-AM in rat hippocampal neurons. Pharmacological experiments using SNAP, 8-Br-cGMP, diazoxide and several inhibitors revealed that the NO/cGMP/Protein kinsase G (PKG) signaling pathway triggers an increase in [Mg2+]i, and that Mg2+ mobilization is due to Mg2+ release from mitochondria induced by mitoKATP channel opening. In addition, Mg2+ release is potentiated by the positive feedback loop including mitoKATP channel opening, mitochondrial depolarization and PKC activation.

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AGROVOC Keywords

fluorescence magnesium mitochondria mitochondria nitric oxide rats

Bibliographic information

Published in

FEBS letters

Volume 587 Issue 16 Pagination 2643 - 2648 ISSN 0014-5793

Publisher

Elsevier B.V.

Other Subjects

Kmg-104; Pkc; Pkg; [ca2+]i; Neurons; Mg2+; Cyclic gmp; Signal transduction; Cgmp-dependent protein kinase; Diazoxide; Hippocampal neuron; Mitokatp channel; Δψm; Ros; [mg2+]i; Protein kinase c; Cell viability

Language

English

Type

Journal Article; Text

In AGRIS since: 2024-02-28

Format: MODS

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Links

DOI DOI https://dx.doi.org/10.1016/j.febslet.2013.06.049

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