Isolation of pigment-free bulk lipids from thylakoids
1993
Bratt, C.E. | Akerlund, H.E.
Lipids from spinach thylakoids were extracted with chloroform/methanol and separated from pigments in a single chromatographic step run at 5 degrees C using silicic acid adjusted to pH 8. The isolated lipid fraction contained essentially the same amounts of individual lipids as in the initial extract. It contained less than 0.1% of the initial chlorophylls and carotenoids.
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