Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs
2019
Pérez-Núñez, Daniel | Sunwoo, Sun-Young | Sánchez, Elena G. | Haley, Nicholas | García-Belmonte, Raquel | Nogal, Marisa | Morozov, Igor | Madden, Daniel | Gaudreault, Natasha N. | Mur, Lina | Shivanna, Vinay | Richt, Juergen A. | Revilla, Yolanda
African swine fever virus (ASFV) causes serious disease in domestic pigs for which there is no vaccine currently available. ASFV is a large DNA virus that encodes for more than 150 proteins, thus making the identification of viral antigens that induce a protective immune response difficult. Based on the functional roles of several ASFV proteins found in previous studies, we selected combinations of ASFV recombinant proteins and pcDNAs-expressing ASFV genes, to analyze their ability to induce humoral and cellular immune responses in pigs.Pigs were immunized using a modified prime-boost approach with combinations of previously selected viral DNA and proteins, resulting in induction of antibodies and specific cell-mediated immune response, measured by IFN-γ ELISpots. The ability of antibodies from pigs immunized with various combinations of ASFV-specific antigens to neutralize infection in vitro, and antigen-specific activation of the cellular immune response were analyzed.
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