Determination of melamine and melamine–Cu(II) complexes in milk using a DNA-Ag hydrocolloid as the sensor
2020
Mu, Wei-Yu | Huang, Pu-Zhen | Chen, Qiu-Yun | Wang, Wei
Simple and sensitive methods are required for the detection of melamine since melamine is harmful to human health. To detect the content of melamine and metal–melamine species in milk products, a new luminescent DNA silver hydrocolloid (AgNC₅₃) was synthesized using a modified C3A-rich aptamer as the template. The emission of AgNC₅₃ at 617 nm is sensitive to both melamine–Cu(II) and melamine, while almost no response to the free Cu(II) ion was detected. Moreover, AgNC₅₃ is used to detect precisely the content of melamine in milk with a detection limit as low as 2.7 × 10⁻⁸ M. Thus, the AgNC₅₃-based fluorescence method is a “label-free” fluorescence technology that can be used for the determination of melamine or the melamine–Cu(II) complex in milk products.
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