Isolation and characterisation of a sucrose: sucrose 1-fructosyltransferase gene from perennial ryegrass (Lolium perenne)

Chalmers, J.; Johnson, X.; Lidgett, A.; Spangenberg, G.

Isolation and characterisation of a sucrose: sucrose 1-fructosyltransferase gene from perennial ryegrass (Lolium perenne)

2003

Chalmers, J. | Johnson, X. | Lidgett, A. | Spangenberg, G.

A sucrose: sucrose 1-fructosyltransferase (1-SST) gene and cDNA (Lp1-SST) from perennial ryegrass (Lolium perenne) were isolated. The Lp1-SST gene was fully sequenced and shown to contain three exons and two introns. Nucleotide sequence analysis of the 4824 bp Lp1-SST genomic sequence revealed 1618 bp of 5' UTR and an open reading frame of 1962 bp encoding a protein of 653 amino acids. Lp1-SST is 95% identical to the tall fescue 1-SST and contains plant fructosyltransferase functional domains. Lp1-SST corresponds to a single copy gene in perennial ryegrass, and is expressed in young leaf bases and mature leaf sheaths. The recombinant Lp1-SST protein from corresponding cDNA expression in Pichia pastoris showed 1-SST activity.

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AGROVOC Keywords

chemistry cloning complementary dna dna dna enzymology gene expression gene expression regulation gene expression regulation genetics grasses lolium lolium perenne metabolism molecular cloning pasture plants phylogeny phylogeny pichia pastoris plant recombinant proteins sequence analysis sequence analysis

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Published in

Journal of plant physiology

Volume 160 Issue 11 Pagination 1385 - 1391 ISSN 0176-1617

Other Subjects

Molecular sequence data; Plant proteins; Hexosyltransferases; Molecular; Recombinant fusion proteins; Sucrose: sucrose 1-fructosyltransferase; Isolation & purification; Plant breeding and genetics; Plant physiology and biochemistry; Amino acid sequence; Komagataella pastoris; 5'-untranslated region; Hexosyltransferases; Nucleotide sequences; Enzymologic; Plant leaves; Plant proteins; Complementary; Molecular sequence data

Language

English

Note

2019-12-05

Type

Journal Article; Text

In AGRIS since: 2024-02-28

Format: MODS

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Isolation and characterisation of a sucrose: sucrose 1-fructosyltransferase gene from perennial ryegrass (Lolium perenne)

2003

AGROVOC Keywords

Bibliographic information