Methylobacterium gossipiicola sp. nov., a pink-pigmented, facultatively methylotrophic bacterium isolated from the cotton phyllosphere
2012
Madhaiyan, Munusamy | Poonguzhali, Selvaraj | Senthilkumar, Murugaiyan | Lee, Jung-Sook | Lee, Keun-Chul
A pink, aerobic, facultatively methylotrophic, motile, Gram-negative rod, designated Gh-105T, was isolated from the phyllosphere of cotton from Coimbatore (Tamilnadu, India). 16S rRNA gene sequence analysis showed clearly that the isolate belonged to the Methylobacterium cluster. Strain Gh-105T was most closely related to Methylobacterium adhaesivum AR27T (99 % 16S rRNA gene sequence similarity) and Methylobacterium iners 5317S-33T (97.5 %). The isolate grew with C1 compounds such as methanol and dichloromethane, but not with formaldehyde, formate, methylamine, trimethylamine or methane, as sole carbon sources and carried mxaF, which encodes methanol dehydrogenase and supports methylotrophic metabolism. The major fatty acid was C18 : 1Ï7c and the G+C content of the genomic DNA was 64.2 mol%. Physiological and biochemical data and DNA–DNA relatedness with M. adhaesivum KACC 12195T and M. iners KACC 11765T revealed clear phenotypic and genotypic differences. For this reason, we propose that strain Gh-105T ( = CCM 7572T = NRRL B-51692T) represents the type strain of a novel species, with the name Methylobacterium gossipiicola sp. nov.
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