Legionella nagasakiensis sp. nov., isolated from water samples and from a patient with pneumonia
2012
Yang, Genyan | Benson, Robert F. | Ratcliff, Rodney M. | Brown, Ellen W. | Steigerwalt, Arnold G. | Thacker, W Lanier | Daneshvar, Maryam I. | Morey, Roger E. | Saito, Atsushi | Fields, Barry S.
A novel Legionella species was identified based on analysis of 16S rRNA and mip (macrophage infectivity potentiator) gene sequences, cellular fatty acids, isoprenoid quinones, biochemical reactions, antigens and quantitative DNA–DNA hybridization. Strain CDC-1796-JAP-ET was isolated from well water at the Nagasaki Municipal Medical Center, Japan. Two strains, CDC-3041-AUS-E and CDC-3558-AUS-E, were isolated from water samples during an outbreak of legionellosis in South Australia. Strain CDC-5427-OH-H was isolated from a 66-year-old female patient diagnosed with Legionnaires’ disease in the US. Cells from these four strains were Gram-negative, non-fluorescent, rod-shaped, and positive for alkaline phosphatase, esterase, leucine arylamidase, catalase, gelatinase, β-lactamase and tyrosine browning assay. Phylogenetic analysis of 16S rRNA and mip genes revealed that the four strains formed a distinct cluster within the genus LEGIONELLA: The bacteria contained branched-chain fatty acids and quinones that are typical of members of the genus LEGIONELLA: Slide agglutination tests demonstrated no cross-reaction with 52 previously described members of the LEGIONELLACEAE: DNA–DNA hybridization studies indicated that DNAs from the four strains were highly related (78–84 %) but they showed 29 % relatedness to Legionella oakridgensis ATCC 33761T and less than 10 % to strains of other Legionella species tested. These characterizations suggest that the isolates represent a novel species, for which the name Legionella nagasakiensis sp. nov. is proposed; the type strain is CDC-1796-JAP-ET ( = ATCC BAA-1557T = JCM 15315T).
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