Structural identification of ginseng polysaccharides and testing of their antioxidant activities
2008
Luo, D. | Fang, B.
A large number of polysaccharides were presented in boiling-water extraction of ginseng. A DEAE-Sepharose CL-6B column chromatography was used to isolate the major polysaccharides from ginseng. Two fractions were obtained, named GPII and GPIII. Both GPII and GPIII were a neutral polysaccharide and a single peak in HPLC with Sugar KS-804 column, with a molecular weight of 3 x 10⁵ and 4 x 10⁵, respectively, comprised mainly of glucose. Analysis by Periodate oxidation-Smith degradation indicated that GP was composed of 60.06% (1 to)- or (1 to 6)-glycosidic linkages and 39.94% (1 to 3)-glycosidic linkages, and GPIII 16.23% (1 to)- or (1 to 6)-glycosidic linkages, 25.98% (1 to 2)-glycosidic linkages, and 57.79% (1 to 3)-glycosidic linkages. On the basis of superoxide radical assay, hydroxyl radical assay and self-oxidation of 1,2,3-phentriol assay, their antioxidant activities were investigated. GPII exhibited equivalent inhibiting power for self-oxidation of 1,2,3-phentriol to Vc, a little higher scavenging activity of superoxide radical and hydroxyl radical than Vc, and should be explored as a novel potential antioxidant.
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