Metabolism of ent-kaurene to gibberellin A12-aldehyde in young shoots of normal maize
1992
Suzuki, Y. | Yamane, H. | Spray, C.R. | Gaskin, P. | MacMillan, J. | Phinney, B.O.
Young shoots of normal maize (Zea mays L.) were used to determine both the stepwise metabolism of ent-kaurene to gibberellin A12-aldehyde and the endogenous presence of the members in this series. Each of the five steps in the sequence was established by feeds of 17-(13)C,(3)H-labeled kauranoids to cubes from the cortex of elongating internodes, to homogenates from the cortex of elongating internodes, and/or to homogenates from dark-grown seedlings. The (13)C-metabolites were identified by Kovats retention indices (KRI) and full-scan capillary gas chromatography-mass spectrometry (GC-MS). Five substrates and the final product in this sequence were shown to be native by the isotopic dilution of 17-(13)C,(3)H-labeled substrates added as internal standards to extracts obtained from elongating internodes. Evidence for the isotopic dilution was obtained by KRI and full-scan capillary GC-MS. Thus, we document the presence in young maize shoots of the metabolic steps, ent-kaurene leads to ent-kaurenol leads to ent-kaurenal leads to ent-kaurenoic acid leads to ent-7 beta-hydroxykaurenoic acid leads to gibberellin A12-aldehyde.
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