Impurity-induced peroxidase mimicry of nanoclay and its potential for the spectrophotometric determination of cholesterol
2016
Aneesh, K. | Vusa, Chiranjeevi Srinivasa Rao | Berchmans, Sheela
A green version of the “Fe” impurity-induced peroxidase mimicry exhibited by simple and cheap substrate “nanoclay (NC)” along with the highly sensitive amperometric and spectrophotometric determination of cholesterol is demonstrated. The “Fe” impurity can act as the catalyst center for hydrogen peroxide reduction similar to the horseradish peroxidase (HRP)-catalyzed reaction. The Michaelis–Menten constant for the NC-catalyzed reaction is found to be lower than that of the HRP-catalyzed reaction indicating high affinity for the substrate. The NC-modulated peroxidase-like catalytic activity originates from the electron transfer between the reducing substrate in the catalyst center and H₂O₂ with the intermediate generation of hydroxyl radicals. The peroxidase mimicry is successfully applied for the low-potential electrochemical detection of H₂O₂ (linear detection range 1.96–10.71 mM, R ² = 0.97). The H₂O₂ sensing platform is further modified with cholesterol oxidase (CHOx) for the spectrophotometric (linear detection range 50–244 μM, R ² = 0.99) and amperometric detection of cholesterol (linear detection range 0.099–1.73 mM, R ² = 0.998). Graphical abstract Peroxidase mimicry of nanoclay for the determination of cholesterol
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