Identification of S-haplotypes of European cultivars of sour cherry
2017
Lisek, Anna | Kucharska, Danuta | Głowacka, Agnieszka | Rozpara, Elżbieta
The sour cherry is known to exhibit the phenomenon of gametophytic self-incompatibility which prevents self-fertilization. In sour cherry, besides self-incompatible cultivars, there often occur self-compatible cultivars. This is due to the occurrence of natural mutations of the S-RNase or SFB genes and, consequently, loss of functionality of S-alleles. Here we present the results of the identification of S-haplotypes of 21 cultivars of sour cherry from various regions of Europe. The analyses were performed using methods based on the amplification of intron I and intron II of the S-RNase gene and fragments specific to the individual alleles of the S-RNase or SFB genes. The tested cultivars were found to contain 15 S-haplotypes: S₁, S₁ʹ, S₄, S₆, S₆ₘ, S₆ₘ₂, S₉, S₁₂, S₁₃, S₁₃ʹ, S₂₆, S₃₅, S₃₆ₐ, S₃₆b, and S₃₆b₂. The most frequently occurring S-haplotypes were S₁₃ʹ (61.9%), S₃₆ₐ (57.1%), and S₂₆ (47.6%). On the basis of the results, 17 of the 21 cultivars were deduced to be self-compatible. The results will be of use in the production of sour cherry fruit by facilitating the selection of suitable pollinating cultivars. The results are also expected to be useful in the breeding of new cultivars of sour cherry when selecting genotypes for crosses.
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