Identification of the Genetic Region of Bacillus subtilis B3 Renders Bacillus subtilis 168 Biosynthesis of Lipopeptide Surfactin Positive
2003
Yao, S.Y. | Gao, X.W. | Fuchsbauer, N. | Hillen, W. | Vater, J. | Wang, J.S.
The 3642 base pairs genomic region of Bacillus subtilis B3, which converts B. subtilis 168 into a producer of surfactin, includes srfDB3 encoding thioesterase, aspB3 encoding aspartate aminotransferase, lpaB3 encoding phosphopantetheinyl transferase and yczEB3 encoding YczE-like protein. Deletion analysis and functionally complement results indicated that truncation of srfDB3, aspB3 and yczEB3 did not affect biosynthesis of surfactin in B. subtilis 168. The lpaB3 gene with its promoter and ribosome-binding site is essential, whereas its transcription terminator is not necessary for biosynthesis of surfactin in B. subtilis 168.
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