Characterisation of related red-berried and white-berried grapevine cultivars
2017
Falco, V. | Castro, I. | Oliveira, A. A. | Ferreira, V. | Martin, J. P. | Ortiz, J. M. | Oliveira, P. | Moura, J. P. | Magalhaes, N. | Pinto-Carnide, O.
Eight presumably related dark red-berried (dR), light red-berried (lR) and white-berried (W) grapevine cultivars grown under similar environmental conditions were characterised on the basis of morphologic (phenotype) and molecular (genotype) parameters. The ampelographic characterisation was based on the main descriptors that correspond to the 'Primary descriptor priority list' suggested by the International Organisation of Vine and Wine (OIV). At the molecular level, the total genomic DNA of the cultivars was isolated from frozen grapevine leaves. Six microsatellite loci were used for characterisation of the grape cultivars: 'VVS2', 'VVMD5', 'VVMD7', 'VVMD27', 'VrZAG62', and 'VrZAG79'. This molecular characterisation allowed the confirmation, in seven of the eight studied cultivars, including dark red, light red and white-berried variants, of colour mutants with origin in three different varieties. 'Pinot Blanc' (W), 'Pinot Gris' (lR) and 'Pinot Noir' (dR) formed a group of colour mutants, and 'Malvasia Fina' (W) and 'Malvasis Fina Roxa' (lR) formed another group. 'Moscatel Galego Branco' (W) and 'Moscatel Galego Roxo' (lR) were confirmed as colour mutants, but their allelic profile was not related to that of 'Moscatel Galego Tinto' (dR).
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