Molecular Marker-Facilitated Analysis of Introgression in Winter Wheat × Triticum tauschii Populations
1995
Fritz, A. K. | Cox, T. S. | Gill, B. S. | Sears, R. G.
Triticum tauschii (Coss.) Schmal., a wild, diploid ancestor of common wheat (Triticum aestivum L.) has become an important genetic resource for wheat improvement. As this trend continues, a detailed understanding of the introgression process becomes increasingly important. This study utilized molecular markers to track introgression of T. tauschii DNA into common wheat. Genotypes of 147 BC₂F₁ lines derived from crosses between wheat and T. tauschii were determined for one protein, 25 restriction fragment length polymorphisms, and eight random amplified polymorphic DNA markers. MAPMAKER and GMENDEL computer programs were used to determine linkage groups and genetic distances. Map orders and recombination distances of linked probes compared favorably with the existing T. tauschii map except for chromosome 4D, which was inherited as a unit. Certain chromosomal segments from either T. aestivum or T. tauschii were transferred more frequently than expected, and choice of recurrent parent played a role in the level of introgression that occurred. Recombination between the D genomes of T. aestivum and T. tauschii occurs at a level similar to that in an intraspecific cross; any abnormalities that exist should not hinder the ability to transfer genes of interest while eliminating flanking chromatin that may be responsible for deleterious effects. Contribution no. 95-47-J from the Kansas Agric. Exp. Stn.
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