Polymorphic microsatellite loci for studies of the Ozark hellbender (Cryptobranchus alleganiensis bishopi)
2009
Johnson, Jarrett R. | Faries, Kaitlyn M. | Rabenold, Jessica J. | Crowhurst, Rachel S. | Briggler, Jeffrey T. | Koppelman, Jeffrey B. | Eggert, Lori S.
The hellbender is the only North American member of the aquatic salamander family Cryptobranchidae and is a species of conservation concern across its range. We developed eight polymorphic microsatellite loci for hellbenders using a magnetic bead enrichment protocol and a PCR-based detection technique. Allelic diversity averaged 4.0 (±1.8 SD) per locus and heterozygosity averaged 0.56 (±0.30 SD). The hellbender is rare and difficult to study due to its cryptic life history. These loci will provide a valuable resource for population studies, which could inform future conservation and management decisions.
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