Suppression of the ptsH mutation in Escherichia coli and Salmonella typhimurium by a DNA fragment from Lactobacillus casei

Monedero, V.; Postma, P.W.; Perez-Martinez, G.

Suppression of the ptsH mutation in Escherichia coli and Salmonella typhimurium by a DNA fragment from Lactobacillus casei

1998

Monedero, V. | Postma, P.W. | Perez-Martinez, G.

A DNA fragment from Lactobacillus casei that restores growth to Escherichia coli and Salmonella typhimurium ptsH mutants on glucose and other substrates of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) has been isolated. These mutants lack the HPr protein, a general component of the PTS. Sequencing of the cloned fragment revealed the absence of ptsH homologues. Instead, the complementation ability was located in a 120-bp fragment that contained a sequence homologue to the binding site of the Cra regulator from enteric bacteria. Experiments indicated that the reversion of the ptsH phenotype was due to a titration of the Cra protein, which allowed the constitutive expression of the fructose operon.

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AGROVOC Keywords

bacterial proteins carbohydrate metabolism cloning dna dna escherichia coli fermentation fermentation fructose fructose gene transfer genetic transformation genetics inhibition kinases lactobacillus casei metabolism mutation salmonella typhimurium salmonella typhimurium sequence analysis sugars transcription factors

Bibliographic information

Published in

Journal of bacteriology

Volume 180 Issue 19 Pagination 5247 - 5250 ISSN 0021-9193

Other Subjects

Molecular; Cra protein; Sequence homology; Amino acid; Molecular sequence data; Genbank/aj006018; Repressor proteins; Nucleotide sequences; Regulator genes; Genetic; Suppression; Genetic complementation test; Molecular sequence data; Escherichia coli proteins; Phosphoenolpyruvate:carbohydrate phosphotransferase; Phosphoenolpyruvate sugar phosphotransferase system; Bacterial; Cra repressor; Phosphotransferases (kinases); Dna-binding proteins; Lacticaseibacillus casei

Language

English

Note

2019-12-05

Type

Text; Journal Article

In AGRIS since: 2024-02-28

Format: MODS

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