Human sperm aneuploidy after exposure to polycyclic aromatic hydrocarbons
2016
Radwan, Michał | Jurewicz, Joanna | Sobala, Wojciech | Brzeźnicki, Sławomir | Radwan, Paweł | Jakubowski, Lucjusz | Hawuła, Wanda | Ulańska, Anna | Hanke, Wojciech
The purpose of this cross-sectional study was to investigate whether environmental exposure to polycyclic aromatic hydrocarbons (PAHs) was associated with sperm aneuploidy. A sample of 181 men who attended an infertility clinic for diagnostic purposes and who had a normal semen concentration of 20–300×106 spermatozoa mL-1 or slight oligozoospermia (semen concentration of 15–20×106 spermatozoa mL-1; WHO 1999) provided urine and semen samples. Analysis of the level of PAH biomarker 1-hydroxypyrene (1-OHP) in urine was performed using high-performance liquid chromatography. Sperm aneuploidy was assessed using multicolour florescence in situ hybridisation (FISH) using DNA probes specific for chromosomes X, Y, 18, 13 and 21. Positive associations were observed between the level of 1-OHP in urine and total sex-chromosome disomy (P=0.03) and chromosome-18 disomy (P=0.03). These results suggest that environmental exposure to PAHs may be associated with sperm aneuploidy. This is the first epidemiological study to investigate the relationship between environmental exposure to PAHs and sperm aneuploidy. Therefore, these findings require further replication in other populations using different biomarkers of PAH exposure.
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