Improved Method for the Determination of Cyanuric Acid in Animal Feed by GC-MS
2009
Tang, Hubert P. O | Lai, Shirley S. L | Lai, Ashley Y. H | Lee, W. O
A specific and sensitive analytical method for the quantitative determination of cyanuric acid in animal feed was developed. Sample preparation involved the diethylamine/acetonitrile/water extraction of feed using sonication and shaking. The extract was subjected to clean-up by dual solid phase extraction using mixed mode anionic and cationic extraction cartridges. After removal of clean-up solvent, cyanuric acid was converted to a tert-butyldimethylsilyl derivative and was determined by gas chromatography-mass spectrometry in the selected ion monitoring mode. ¹³C ₃ ¹⁵ N₃-cyanuric acid was employed as the internal standard. The calibration curve was found to be linear up to 4 mg kg⁻¹. LOD and LOQ were determined to be 0.06 to 0.4 mg kg⁻¹ for fish and chicken feed. The mean recovery of cyanuric acid was 96 to 98% with relative pooled standard deviation of 1.8-7.4% in the range of 0.5 to 100 mg kg⁻¹ for fish and chicken feed. The validated method was applicable for analysing cyanuric acid in animal feed.
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