Development of a chemiluminescent enzyme immunoassay to detect cadmium in cereal samples
2018
Xiao, Zhi-Li | Tian, Wei | Xu, Zhen-Lin | Sun, Yuan-Ming
In this study, we develop a sensitive indirect competitive chemiluminescent enzyme immunoassay (ic-CLEIA) to detect cadmium (Cd) in cereals. A luminol-hydrogen peroxide chemiluminescence system catalysed by horseradish peroxidase (HRP) was used as the signal detecting system. The method showed a half-maximal inhibition concentration (IC₅₀) of 0.5 ng/mL and a limit of determination (LOD) of 0.06 ng/mL. The assay indicated an effective linear range from 0.16 to 1.56 ng/mL. There were no obvious cross-reactivities with other metal ions, including Hg²⁺, Pb²⁺, As²⁺, Ag⁺, Fe³⁺, Cu²⁺, Cr²⁺, Au³⁺, Ca²⁺, Mg²⁺, Mn²⁺ and Zn²⁺. Recovery rates ranged from 79.2% to 120.0% for rice, maize, wheat, brown rice and glutinous rice samples. Twenty-five practical cereal samples were analysed, and the comparative analysis of the results showed a strong correlation (r = 0.984) between the CLEIA and the GFAAS methods. The results indicated that the ic-CLEIA developed was acceptable to screen for cadmium in cereal samples.
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