Considerations for cholinesterase biomonitoring in flower and ornamental plant greenhouse workers

Flower cultivation in greenhouses enhances productivity and ensures high-quality product supply throughout the year. However, under such protected environments, climate conditions are designed to favor flower growth and can intensify occupational exposure to pesticides. In this study, flower greenhouse workers were tested for cholinesterase inhibition, a valuable indicator of occupational exposure to organophosphate and carbamate pesticides. The quantified inhibition was also associated with working practices using these outcomes to explore ways in which provisions of regulatory Occupational Health Control Programs can be met considering the peculiarities of this working environment. Despite their preliminary nature, and considering that workers had not been previously tested, the obtained results suggested that the designed pre- and post-screening process, enabled biomonitoring of the exposed population. The pre-exposure sample was collected after 11 days from pesticide spraying and the post-exposure sample was collected no later than 10 days after pesticide spraying. AChE activity was significantly depressed in flower greenhouse workers, comparing pre- and post-exposure intervals. Nursery workers, often overlooked, presented greater inhibition of AChE activity. Such finding is consistent with effects believed to be associated with the described job and the way activities are conducted, once nursery workers are regularly exposed to low levels of pesticides owing to their continuous contact with sprayed crops. As predicted by Occupational Safety and Health Standards, establishing biomonitoring programs is achievable, despite the intensive use of pesticides. Quantification is important to target the exposed population but is not sufficient to assure good working conditions and avoid exposure. Administrative and working practices, such as job misclassification, non-compliance with national regulations for occupational health control, pesticide handling, lack of REI protocols, and unsuitable delivery of PPE, must be addressed. Anything less will contribute to worsening an already bleak picture in terms of occupational safety and health in flower and ornamental plant greenhouses.