Use of 4-Amino-3,5,6-trichloropicolinic Acid as an Auxin Source in Plant Tissue Cultures
1978
Colins, G. B. | Vian, W. E. | Phillips, G. C.
The auxin-like properties of 4-amino-3,5,6-trichloropicolinic acid (picloram) were compared with those of other auxins in tissue cultures of wheat (Triticum aestivum), soybeans (Glycine max), tobacco (Nicotiana tabacum), and jackbean (Canavalia ensiformis) explants. Certain problems associated with the use of 2,4-dichlorophenoxyacetic acid (2,4-D) in tissue culture were not encountered when picloram was substituted for 2,4-D in the culture medium of 2,4-D-requiring plant materials. Picloram performed as well or better than the auxins to which it was compared when used in plant tissue cultures. In general, picloram was more effective at low concentrations than the other auxins. The data indicate potential uses of picloram in routine callus cultures, in regeneration of plants from calli, and in research concerning the physiological development of plant tissues.
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