Biodegradation of triphenyl phosphate using an efficient bacterial consortium GYY: Degradation characteristics, metabolic pathway and 16S rRNA genes analysis
2020
Yang, Yuanyu | Yin, Hua | Peng, Hui | Lu, Guining | Dang, Zhi
Triphenyl phosphate (TPHP) was frequently detected in various environment, which has caused wide attention out of its adverse effects on organisms. Hence, an effective and reasonable method is in urgent demand for removing TPHP. In this study, microbial consortium GYY with efficient capacity to degrade TPHP has been isolated, which could degrade 92.2% of TPHP within 4 h under the optimal conditions (pH 7, inoculum size 1 g/L wet weight, 30 °C, TPHP initial concentration 3 μmol/L). Some intermediate products such as diphenyl phosphate (DPHP), phenyl phosphate (PHP), OH-TPHP, and methoxylation products were identified, suggesting that TPHP was metabolized by hydrolysis, methoxylation after hydrolysis, and methoxylation after hydroxylation pathways. The sequencing analysis demonstrated that Pseudarthrobacter and Sphingopyxis were the dominant genera in consortium GYY during the process of TPHP biodegradation. Also, Sphingopyxis (GY-1) that degraded 98.9% of TPHP (3 μmol/L) within 7 days was further isolated and identified. Overall, this study provides a new insight on TPHP metabolic transformation by consortium and theoretical basis of developing bioremediation technology for TPHP contamination.
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