Somatic embryogenesis and organogenesis induced on the immature zygotic embryo of sunflower (Helianthus annuus L.) cultivated in vitro: role of the sugar
1995
Jeannin, G. | Bronner, R. | Hahne, G.
Immature zygotic embryos of sunflower constitute an experimental system where the change of a single key factor (sucrose concentration) conditions the in vitro morphogenesis to either organogenesis (87 mM sucrose) or somatic embryogenesis (350 mM sucrose) Experiments with a variety of culture media differing in the sugar type and concentration, as well as osmotic pressure, indicate that a minimal threshold level of both, sugar supply and osmotic pressure, are required for somatic embryogenesis, but not organogenesis, to occur. The nature of the sugar used, though, was less important.
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