Relative immunogenicity of beta-lactoglobulin, bovine serum albumin, and model acid whey product
1997
Heddleson, R.A. | Allen, J.C.
The relative immunogenicities of bovine beta-1actoglobulin (beta-LG) and bovine serum albumin (BSA) were investigated using a rat model system. ELISAs were developed to quantify antibody titers in Sprague-Dawley rats injected intraperitoneally with dosages of beta-LG ranging from 0.01 to 1000 micrograms, and dosages of BSA ranging from 0.1 to 1000 micrograms. Primary IgG and IgE responses to BSA increased with increasing dosage of BSA, with a minimum of 100 and 1000 micrograms (respectively) necessary for responses significantly (P < 0.05) greater than that of untreated controls. Secondary (boosted) IgE responses to BSA were not significantly P < 0.05) greater than pimary responses. Greater specific IgG and IgE responses to beta-LG occurred at 3 than 2 weeks post-injection, and at lower dosages (0.1 or 1.0 micrograms). Compared over a range of dosages, the immunogenicities of BSA and beta-LG did not differ significantly (P > 0.05). The results of this study may aid in developing operating parameters for affinity absorption methods that selectively remove allergenic proteins from milk or acid whey.
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