Exo- and Endocytotic trafficking of SCAMP2
2009
Toyooka, Kiminori | Matsuoka, K. (Ken)
Exo- and endocytotic membrane trafficking is an essential process for transport of secretory proteins, extracellular glycans, transporters and lipids in plant cells. Using secretory carrier membrane protein 2 (SCAMP2) as a marker for secretory vesicles and tobacco BY-2 cells as a model system, we recently demonstrated that SCAMP2 positive structures containing secretory materials are transported from the Golgi apparatus to the plasma membrane and/or cell plate. This structure is consisted with clustered vesicles and was thus named the secretory vesicle cluster (SVC). Here, we have utilized the reversible photo-switching fluorescent protein Dronpa¹ to trace the movement of SCAMP2 on the PM and cell plate. Activated SCAMP2-Dronpa fluorescence on the plasma membrane and cell plate moved into the BY-2 cells within several minutes, but did not spread around plasma membrane. This is consistent with recycling of SCAMP2 among endomembrane compartments such as the TGN, plasma membrane and cell plate. The relationship between SVC-mediated trafficking and exo- and endocytosis of plant cells is discussed taking into account this new data and knowledge provided by recent reports.
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