Determination of alditols by capillary electrophoresis with indirect laser-induced fluorescence detection
2015
Xiao, Yao | Li, Yunfang | Ying, Jinjin | Tian, Yu | Xiao, Yuxiu | Mei, Zhinan
A novel method was developed based on capillary electrophoresis with indirect laser-induced fluorescence detection for the determination of alditols including xylitol, sorbitol, arabinitol and mannitol. The baseline separation was achieved with a capillary with 30μm inner diameter and running buffer (pH 9.6) composed of 40mM sodium tetraborate, 5% (v/v) methanol, 10−5M sodium fluorescein as background fluorescence. Calibration curves (0.2–2.0mgmL−1) showed good linear correlations (r>0.999). The detection limits of the alditols were in the range of 19.0–24.4μgmL−1. The intra-day and inter-day RSDs for migration times and peak areas were less than 1.6% and 4.3%, respectively. The average recoveries were in the range of 99.6–105.5%. The developed method was successfully applied to determination of alditols in different real samples, and the results were compared with those of capillary electrophoresis with indirect ultraviolet detection method.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by National Agricultural Library