Electrochemical Sensor for Lead Cation Sensitized with a DNA Functionalized Porphyrinic Metal–Organic Framework
2015
Cui, Lin | Wu, Jie | Li, Jie | Ju, Huangxian
An efficient electrochemical sensor was presented for lead cation detection using a DNA functionalized iron–porphyrinic metal–organic framework (GR–5/(Fe–P)ₙ-MOF) as a probe. The newly designed probe showed both the recognition behavior of GR–5 to Pb²⁺ with high selectivity and the excellent mimic peroxidase performance of (Fe–P)ₙ-MOF. In the presence of Pb²⁺, GR–5 could be specifically cleaved at the ribonucleotide (rA) site, which produced the short (Fe–P)ₙ-MOF-linked oligonucleotide fragment to hybridize with hairpin DNA immobilized on the surface of screen-printed carbon electrode (SPCE). Because of the mimic peroxidase property of (Fe–P)ₙ-MOF, enzymatically amplified electrochemical signal was obtained to offer the sensitive detection of Pb²⁺ ranging from 0.05 to 200 nM with a detection limit of 0.034 nM. In addition, benefiting from the Pb²⁺-dependent GR–5, the proposed assay could selectively detect Pb²⁺ in the presence of other metal ions. The SPCE based electrochemical sensor along with the GR–5/(Fe–P)ₙ-MOF probe exhibited the advantages of low-cost, simple fabrication, high sensitivity and selectivity, providing potential application of on-site and real-time Pb²⁺ detection in complex media.
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