Protein Corona Analysis of Silver Nanoparticles Exposed to Fish Plasma
2017
Gao, Jiejun | Lin, Lu | Wei, Alexander | Sepulveda, Maria S.
Nanoparticles (NPs) in contact with biological fluids experience changes in surface chemistry that can impact their biodistribution and downstream physiological impact. One such change involves the formation of a protein corona (PC) on the surface of NPs. Here we present a foundational study of PC formation following the incubation of polyvinylpyrrolidone-coated AgNPs (PVP-AgNPs, 50 nm) in the plasma of smallmouth bass (Micropterus dolomieu). The level of PC formation increases with exposure time and is also affected by gender, with AgNPs incubated in male plasma having PCs slightly thinner than and ζ potentials less negative than those of AgNPs incubated in female plasma. Proteomic analysis also revealed gender-specific differences in PC composition: in particular, egg-specific proteins (vitellogenin and zona pellucida) were identified in only PCs derived from female plasma, raising the possibility of their roles in AgNP-related reproductive toxicity by promoting their accumulation in developing oocytes.
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