Influence of precursors on production of isoquinoline alkaloids in tissue cultures of Cephaelis ipecacuanha
1994
Veeresham, C. | Kokate, C. | Venkateshwarlu, V.
The exogenous feeding of precursors e.g. shikimic acid and L-phenylalanine (50 mgl-1) influenced the overall bioproduction of cephaeline in callus cultures of Cephaelis ipecacuanha. The concentration of cephaeline was practically doubled in the medium (0.109% mg) and callus (0.035% mg) following supplementation with shikimic acid (50 mgl-1). Likewise the addition of L-phenylalanine (50 mgl-1) also enhanced cephaeline production both in the medium (0.075% mg) and callus (0.003%). However, neither shikimic acid nor phenylalanine had any real effect on emetine production. The exogenous feeding of L-phenylalanine (50 mgl-1) and shikimic acid (50 mgl-1) to suspension cultures of C. ipecacuanha influenced bioproduction of cephaeline, which was detected in appreciable quantities only in the medium, while the biosynthesis of emetine remained unaffected. The cephaeline content was increased to 0.129% mg from 0.076% mg in control cultures on treatment with shikimic acid (50 mgl-1), while phenylalanine enhanced bioproduction up to 0.13% mg. The supplementation of L-phenylalanine and shikimic acid to immobilized cell cultures significantly influenced bioproduction of cephaeline to the extent of 1.068% and 1.093% mg, respectively, over the control cultures (0.585% mg). Both precursors failed to influence emetine production in callus, cell and immobilized cultures of Cephaelis ipecacuanha.
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