Real-Time Fluorometric Assay for Acetylcholinesterase Activity and Inhibitor Screening through the Pyrene Probe Monomer–Excimer Transition
2013
Chen, Jian | Liao, Dongli | Wang, Yan | Zhou, Huipeng | Li, Wenying | Yu, Cong
A choline labeled pyrene probe (Py-Ch) was designed and synthesized. Poly(vinylsulfonate) (PVS) could induce Py-Ch aggregation. The aggregation and deaggregation process could be finely controlled by the acetylcholinesterase (AChE) enzymatic hydrolysis of Py-Ch. The resulting excimer–monomer transition provided a facile way for real-time AChE activity fluorometric assay and inhibitor screening.
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