Enrichment of erucic acid from crambe oil in a recirculated packed bed reactor via lipase-catalyzed ethanolysis
2013
No, Da Som | Zhao, Tingting | Kim, Byung Hee | Choi, Hee-Don | Kim, In-hwan
Erucic acid enrichment from crambe oil was accomplished in a recirculated packed bed (RPBR) reactor via ethanolysis using Novozym 435 lipase from Candida antarctica as a biocatalyst. The content of erucic acid of the crambe oil used was 59.8 mol% The erucic acid was located predominantly in the sn-1,3 position of the triacylglycerol of the crambe oil. Novozym 435 used in this study has selectivity toward fatty acids in sn-1,3 position of triglyceride in excess ethanol. The effects of reaction temperature, molar ratio, and residence time of the substrate in RPBR on the enrichment of erucic acid as a function of reaction time were studied. The optimal temperature, molar ratio (crambe oil to ethanol), and residence time of the substrate in RPBR were 45°C, 1:60, and 4min, respectively.
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