Effect of Copper on the Expression of TGF-β in Incubated Chondrocytes of Newborn Pigs
2011
Zhu, Xiaoyan | Wang, Jianguo | Xie, Guanghong | Feng, Haihua | Li, Xiaobing | Liu, Lei | Wang, Xiaoxu | Li, Dongna | Liu, Zhaoxi | Qian, Jian | Wang, Zhe | Liu, Guowen
This experiment was conducted to measure the effect of copper supplementation on TGF-β gene expression in chondrocytes of newborn pigs. Chondrocytes were cultured in media containing 15% fetal calf serum supplemented with 0, 15.6, 31.2, and 62.5 μmol/L copper in 90-mm culture plate. Total RNA was isolated from chondrocytes, and TGF-β cDNA was synthesized, amplified, and sequenced. The expression level of TGF-β was examined by reverse transcription polymerase chain reaction. The results showed that the sequence of the cloned TGF-β gene was 99.4% identical to that in GenBank. The expression of TGF-β increased in culture media added with final concentration of 15.6, 31.2, and 62.5 μmol/L copper. In this study, the optimal copper concentration and optimal culture time for the highest level of TGF-β expression were 31.2 μmol/L and 48 h, respectively.
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