Polymorphisms identification and associations of KLF7 gene with cattle growth traits
2011
Ma, L. | Qu, Y.J. | Huai, Y.T. | Li, Z.J. | Wang, J. | Lan, X.Y. | Zhang, C.L. | Wang, J.Q. | Chen, H.
The Kruppel-like factor KLF7 gene was studied as a candidate for genetic markers of growth traits. We investigated the growth traits at different ages of 1002 Chinese cattles belonging to four breeds and applied artificially created restriction site (ACRS)-PCR-RFLP and DNA sequencing methods to reveal three SNPs: NC_007300:g.41401C>T (p.F54F), 42025T>C and 42075A>G. The statistical results indicated there was a strong linkage not only between C41401T and T42025C in Jiaxian, but also between T42025C and A42075G in Qinchuan, Nanyang and Chinese Holstein respectively (r²>0.33). Significant statistical differences in genotypic frequencies at the three loci displayed that the polymorphisms were remarkably correlated with cattle breeds by χ² test (P<0.001). In addition, the cattle with genotype T₂T₂, C₂C₂ (T42025C), A₃A₃ (A42075G), haplotype C₁C₁C₂C₂A₃A₃ and C₁T₁T₂C₂A₃G₃ showed better growth traits (P<0.01). The biochemical and physiological functions, together with the results obtained in our investigation, suggest that the KLF7 gene might play important roles affecting growth traits in cattle.
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