Effect of NAD⁺-dependent formate dehydrogenase on anaerobic respiration of Shewanella oneidensis MR-1
2013
Mordkovich, N. N. | Voeikova, T. A. | Novikova, L. M. | Smirnov, I. A. | Il’in, V. K. | Soldatov, P. E. | Tyurin-Kuz’min, A. Yu | Smolenskaya, T. S. | Veiko, V. P. | Shakulov, R. S. | Debabov, V. G.
An expression plasmid was constructed in order to carry out heterologous expression of the gene of the NAD⁺-dependent formate dehydrogenase (FDH) from methylotrophic bacterium Moraxella sp. in the cells of Shewanella oneidensis MR-1 under aerobic and anaerobic conditions. In both modes of cell cultivation, recombinant FDH activity was revealed in the cell lysate of the transformants. In the medium with lać tate as a carbon source, the rate of anaerobic respiration determined as the rate of conversion of fumarate (the electron acceptor) to succinate was higher in the transformant with recombinant FDH. Anaerobic cultivation of the FDH-containing transformant of S. oneidensis MR-1 in a microbial fuel cell (MFC) revealed increased current density.
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