Enzymatic modular synthesis and microarray assay of poly-N-acetyllactosamine derivatives
2020
Chen, Congcong | Wang, Shuaishuai | Gadi, Madhusudhan Reddy | Zhu, He | Liu, Fei | Liu, Chang-Cheng | Li, Lei | Wang, Fengshan | Ling, Peixue | Cao, Hongzhi
A facile enzymatic modular assembly strategy for the preparative-scale synthesis of poly-N-acetyllactosamine (poly-LacNAc) glycans with varied lengths and designed sialylation and/or fucosylation patterns is described. These glycans were printed as a microarray to investigate their interactions with a panel of glycan binding proteins (GBPs). Binding affinities revealed that the avidity of GBPs could be largely affected by the length and the patterns of sialylation and fucosylation.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by National Agricultural Library
