The porcine tumor necrosis factor-encoding genes: sequence and comparative analysis

Kuhnert, P.; Wuthrich, C.; Peterhans, E.; Pauli, U.

The porcine tumor necrosis factor-encoding genes: sequence and comparative analysis

1991

Kuhnert, P. | Wuthrich, C. | Peterhans, E. | Pauli, U.

We have cloned and sequenced a 10.22-kb fragment of the genomic locus of the procine tumor necrosis factor-encoding genes, TNF-alpha and TNF-beta. A liver genomic DNA library, partially digested with Sau3AI, was cloned into the phage lambda EMBL4 and screened with a porcine TNF-alpha cDNA probe. Analysis showed that both the TNF-alpha and TNF-beta genes were present on the cloned fragment. In addition, the cloned fragment contained about 2 kb of repetitive sequences 5' to the TNF-beta gene. The TNF genes are arranged in a tandem repeat, as is the case for the human, mouse and rabbit TNF genes. The comparison of both genes with their human homologues displayed a considerable degree of conservation (80%), suggesting an equal evolution rate.

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AGROVOC Keywords

animals cloning dna exons gene expression regulation genes genetics introns necrosis repetitive sequences repetitive sequences swine swine

Bibliographic information

Published in

Gene

Volume 102 Issue 2 Pagination 171 - 178 ISSN 0378-1119

Other Subjects

Nucleic acid; Molecular sequence data; Base sequence; Nucleotide sequences; Sequence homology; Nucleic acid; Molecular; Hemorrhagic fever; Molecular sequence data; Tumor necrosis factor-alpha; Restriction mapping

Language

English

Note

2019-12-04

Type

Journal Article; Text

In AGRIS since: 2024-02-28

Format: MODS

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