In Vivo Intracellular pH Measurements in Tobacco and Arabidopsis Reveal an Unexpected pH Gradient in the Endomembrane System
2013
Martinière, Alexandre | Bassil, Elias | Jublanc, Elodie | Alcon, Carine | Reguera, Maria | Sentenac, Hervé | Blumwald, Eduardo | Paris, Nadine
This work combines biochemistry and live-cell imaging to perform in vivo pH measurements of different compartments of the endomembrane system in plant cells. It reports that the prevacuolar compartments are more alkaline than the trans -Golgi network and provides evidence that endosomal proton pumps and proton efflux transporters contribute to pH homeostasis.
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