Comparative thermostability analysis of zoonotic and human influenza virus A and B neuraminidase
2020
Evseenko, Vasily A. | Svyatchenko, Svetlana V. | Kolosova, Natalia P. | Kovrizhkina, Valentina L. | Marchenko, Vasiliy Y. | Durymanov, Aleksander G. | Goncharova, Natalia I. | Ryzhikov, Alexander B.
Neuraminidase (NA) thermostability of influenza A and B viruses isolated from birds, swine and humans was measured to evaluate its variability associated with host body temperature. The highest 50% inactivation temperature (IT₅₀) was observed with H3N8 avian influenza virus (74 °C), and the lowest IT₅₀ was observed with the seasonal human H3N2 virus (45.5 °C). The IT₅₀ values of A(H1N1)pdm09 viruses 56.4-58.5 °C were statistically higher than that of the prepandemic strain A/Solomon Islands/03/06 (52.5 °C). An analysis of Ca²⁺ binding sites revealed the correspondence of amino acid changes to NA thermostability. This study demonstrates that changes in NA thermostability correspond to differences in host body temperature.
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