Seed germination and phytochemical evaluation in seedlings of Aloe arborescens Mill
2014
Kulkarni, Manoj G. | Amoo, Stephen O. | Kandari, Laxman S. | Van Staden, Johannes
Many Aloe species are exploited as natural products. Generally, the leaves are unsustainably picked from wild plants to meet the market demand. Basic scientific information on seed biology and the ways of increasing levels of secondary metabolites in seedlings is still lacking for Aloe species. This study investigated seed germination requirements and evaluated levels of secondary metabolites in seedlings of Aloe arborescens , an important species in traditional medicine. The highest percentage germination (78%) and the fastest germination rate (GR) (10% d ⁻ ¹) with a mean germination time (MGT) of 9 days were achieved at 20°C under a 16-h photoperiod. At 25°C, maximum percentage germination (67%) (P < 0.05), higher GR (13% d ⁻ ¹) and shorter MGT (6 days) were obtained under constant light. These results indicate that temperature and light play a significant role in germination of A. arborescens seeds. Increasing osmotic pressure on seeds decreased percentage germination, whereas buffering the solution to a range of pH values (4–10) did not significantly affect germination. Smoke–water (1:500 v/v), smoke-isolated karrikinolide (10 ⁻ ⁸ and 10 ⁻ ⁹ M) and potassium nitrate (10 ⁻ ³ and 10 ⁻ ⁴ M) significantly promoted germination compared with the control at 25°C (supra-optimal temperature) under a 16-h photoperiod. These treatments were also effective in increasing secondary metabolite levels (flavonoids and phenolics) in A. arborescens seedlings.
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