Role of cysteine in the formation of 2-methyl-3-furanthiol in a thiamine-cysteine model system
1994
Bolton, T.A. | Reineccius, G.A. | Liardon, R. | Huynh Ba, T.
Labeled 34S-cysteine was used to determine the amount of sulfur from cysteine incorporated into 2-methyl-3-furanthiol (MFT) in thermally processed thiamine/cysteine-glucose/xylose model systems. Four (I-IV) model systems comprised of water, monosodium glutamate, NaCl, inosine-5'-monophosphate, D-glucose, thiamine HCl(I,II,IV), cysteine HCl(I), 34S-cysteine HCl(II-IV), and D-xylose(III,IV) were reacted at 120 degrees C for 1 hr in sealed glass vials. MFT was recovered from processed samples by direct extraction with CH2Cl2 and subsequent concentration under N2 gas. Analysis was performed by gas chromatography/mass spectrometry-selected ion monitoring and MFT was quantified by an internal standard method. The amounts of MFT formed in model systems I, II and IV ranged from 389 to 489 ng/33.3 g model system. No MFT was detected in system III. Only 7.5 to 8.0% of the MFT detected in systems II and IV contained 34S.
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