Antioxidant and cytotoxicites of Pleurotus eryngii residue polysaccharides obtained by ultrafiltration
2016
Ma, Gaoxing | Yang, Wenjian | Fang, Yong | Ma, Ning | Pei, Fei | Zhao, Liyan | Hu, Qiuhui
Response surface methodology was used to optimize the extraction of polysaccharides Pleurotus eryngii residue (CPPS). The yield of CPPS was 10.69 ± 0.31% under the modified condition (leaching temperature of 70 °C, leaching time of 140 min and ratio of water to raw material of 25 mL/g). Ultrafiltration technology was used for further purification of polysaccharides and two fractions of CPPS-1 and CPPS-2 with different molecular weight ranges were obtained. The antioxidant and cytotoxicities of CPPS-1, CPPS-2 and CPPS were determined in vitro. The results showed that CPPS-1 had the strongest activity. Western blotting results showed that the expression of tumor suppressor p53 and apoptosis activator Bax were up-regulated by CPPS-1 (PEPE-3) fraction while the expression of Bcl-2 was down-regulated. Our results suggested that the antitumor activity of PEPE-3 may be related to its capability in inducing apoptosis via activation of mitochondria apoptosis pathway.
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