Analysis of buserelin in urine by online combination of capillary zone electrophoresis with electrospray mass spectrometry
2010
Staňová, Andrea | Marák, Jozef | Maier, Vítězslav | Ranc, Václav | Znaleziona, Joanna | Ševčík, Juraj | Kaniansky, Dušan
A fast and precise analysis of the synthetic peptide buserelin in urine using CZE‐ESI‐MS method has been demonstrated. Formic acid at 50 mmol/L concentration served as backgroud electrolyte in CZE stage and it is compatible with MS detection in positive ionization mode. Two injection modes were tested, i.e. pressure (50 mbar for 5 s) and electrokinetic injection (5 kV for 5 s), of which electrokinetic injection provided better calibration parameters. Buserelin LODs were 0.47 μg/mL in water and 0.63 μg/mL in ten times diluted urine samples using pressure injection, while they were 0.32 μg/mL in water and 0.34 μg/mL in ten times diluted urine samples using electrokinetic injection. Repeatability of buserelin migration times was below 6% (pressure injection mode) and 1% (electrokinetic injection mode). Repeatability of buserelin peak area in SIM mode (m/z=620.5±0.5) was less than 12% (pressure injection mode) and 5.8% (electrokinetic injection mode). In this work, no interferences were observed during the analyses of spiked urine samples.
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