Determination of Alkaloids and Flavonoids in Sophora flavescens by UHPLC-Q-TOF/MS
2021
Dong, Yaqian | Jia, Guoxiang | Hu, Jingwen | Liu, Hui | Wu, Tingting | Yang, Shenshen | Li, Yubo | Cai, Ting
This study is based on UHPLC-Q-TOF/MS and fragment ions to achieve classification and identification of alkaloids and flavonoids in Sophora flavescens. By reviewing the available and relevant literature, the mass fragmentation rules of alkaloids and flavonoids were summarized. 0.1% formic acid water (A) and acetonitrile (B) were used as mobile phases. 37 chemical constituents were identified, including 13 alkaloids and 24 flavonoids. This research method offers a complete strategy based on the fragmentation information of characteristic fragment ions and neutral loss obtained by MS/MS to characterize the chemical composition of Sophora flavescens.
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