Fingerprinting and genetic variability in drumstick (Moringa oleifera Lam.) elite trees using RAPD markers- An underutilized and wild edible future crop
2022
RS, Drisya Ravi | Nair, Bindu R. | Siril, E.A.
The boom in aliment engendered and quality of food is imperative to sustain the growing human population. The availability of diverse germplasm of any crop is a consequential genetic resource to aliment security. In the present study, we used 27 RAPD markers to resolve diversity among 23 superior drumstick genotypes mostly collected from Karnataka, Tamilnadu, and Kerala, states of India. We observed that RAPD markers are highly polymorphic, and among the 519 bands obtained, 471 (89.61%) were polymorphic. The discriminatory power of these markers was determined using various parameters like; polymorphism information content (PIC), marker index (MI), and resolving power (Rp). Primer RAPD-3 showed the highest PIC value (0.35), MI (10.28), and Rp (21.65). The dendrogram generated with UPGMA cluster analysis grouped genotypes into two main clusters with various degrees of sub clustering within the cluster. A high level of genetic diversity was observed within the genotypes. These findings can be further used for the breeding as well as conservation programs of Moringa oleifera.
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