Amine functionalized TiO₂ coated on carbon nanotube as a nanomaterial for direct electrochemistry of glucose oxidase and glucose biosensing
2011
Tasviri, Mahboubeh | Rafiee-Pour, Hossain-Ali | Ghourchian, Hedayatollah | Gholami, Mohammad R.
A nano-composite material consisting of amine functionalized TiO₂-coated carbon nanotubes was prepared and used for glucose oxidase (GOx) absorption. The GOx bearing nanomaterial was fixed on a glassy carbon electrode to construct a novel biosensor for glucose determination. The direct electrochemistry of immobilized GOx and its electron transfer parameters at the modified glassy carbon electrode were reported. The apparent heterogeneous electron transfer rate constant (kₛ) of GOx was estimated to be 3.5s⁻¹, which is higher than those reported previously. Amperometric detection of glucose resulted in a rapid (3s) and stable response in the linear concentration range from 1.8 to 266μM. The apparent Michaelis–Menten constant was measured to be 8.59mM, indicating that the immobilized GOx on NH₂–TiO₂–CNT matrix retained its native activity. The anti-interference ability and long-term stability of the biosensor were also assessed.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by National Agricultural Library
